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Investigate
of Albumin-Porous Holow Fe3O4 Nanoparticles for Dual Drug
Delivery
TABLE
OF CONTENT
LIST
OF FIGURES
LIST
OF ABBREVIATIONS
PART
I. INTRODUCTION
1.1.
Research rationale
1.2.
Research’s objectives
1.3.
Research questions
1.4.
Limitations
1.5.
Definitions
PART
II. LITERATURE REVIEW
2.1.
Cancer Problem
2.2.
Photodynamic Therapy
2.3.
Nanoparticles in biomedical application
2.4.
Protein coated nanoparticles
2.5.
Albumin coated nanoparticles
2.6.
Bovine Serum Albumin (BSA)
2.7.
Porous hollow Fe3O4 nanoparticles
2.8.
Cancer treatment Drugs
2.8.1.
DOXorubicin
2.8.2.
Protoporphyrin IX
PART
III. MATERIALS AND METHODS
3.1.
Materials
3.2.
Treatments
3.3.
Procedure
3.4.
Experiment 1: Characterization of nanocarrier
3.4.1.
Particle Size and Surface charge Measurement
3.4.2.
Encapsulation Efficiency Measurements
3.5.
Experiment 2: Cytotoxic assay
3.5.1.
Dark toxicity
3.5.2.
Light-induced toxicity
PART
IV. RESULTS
4.1.
Particle size and Surface charge
4.2.
Efficiency of Drugs Loading
4.3.
Cytotoxic assay
4.3.1.
Dark toxicity
4.3.2.
Light-induced Toxicity
PART
V. DISCUSSION AND CONCLUSION
5.1.
Discussion
5.1.1.
Particle Size and Surface charge
5.1.2.
Efficiency Drugs loading
5.1.3.
Cytotoxic Assay
5.1.3.1.
Dark Toxicity
5.1.3.2.
Light-induced Toxicity
5.2.
Conclusion
REFERENCES
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