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A new interfacial cross linking technique preparation, characterization and evaluation of calcium alginate nanoparticles as a protein delivery system
Contents
Abstract
Acknowledgements
1 Introduction to Nanoaprticles
1.1 Nanopcapsules
1.1.1 Preparation of Nanocapsules
1.1.2 Applications of Nanocapsules
1.2 Nanospheres
1.2.1 Preparation of Nanospheres
1.2.2 Applications of Nanospheres
1.3 Other types of Nanoparticles
1.4 Polymeric nanoparticles
1.4.1 Physical properties of Natural polymers
1.5 Characterization of Nanoparticles
1.6 Techniques used for modification of surface properties of Nanoparticles
1.7 Therapeutic Applications of Nanoaprticles
1.8 Other uses of Nanoparticles
2 Instrumentation
2.1 Introduction to Instrumentation
2.2 Dynamic Light Scattering
2.2.1 Introduction
2.2.2 Principle
2.2.3 Instrumentation
2.3 Zeta Potential
2.3.1 Introduction
2.3.2 Basic principle and Instrumentation
2.4 Scanning Electron Microscope
2.4.1 Introduction
2.4.2 Basic principles and Instrumentation
2.5 Transmission Electron Microscopy
2.5.1 Introduction
2.5.2 Basic principle and Instrumentation
2.6 Differential Scanning Colorimetry
2.6.1 Introduction
2.6.2 Basic principle and Instrumentation
2.7 Sodium Dodecyl Sulphate -PolyAcryl amide Gel Electrophoresis
2.7.1 Introduction
2.7.2 Basic principle and Instrumentation
2.8 Confocal Laser Scanning Microscope
2.8.1 Introduction
2.8.2 Basic principle and Instrumentation
3 Materials and Materials
3.1 Materials
3.1.1 Sodium Alginate
3.1.2 Calcium Chloride
3.1.3 Diotcyl Sodium Sulposuccinate
3.1.4 Bovine Serum Albumin
3.1.5 Cyclohexane
3.1.6 Ethanol
3.1.7 Albumin FITC
3.1.8 Chitosan
3.2 Methods
3.2.1 Formulation of nanoparticles
3.2.1A) Preparation of Nanoparticles
3.2.1B) Bovine Serum Albumin formulation
3.2.1C) Preparation of FITC labelled BSA nanoparticles
3.2.1D) Preparation of Chitosan caoted Ca-Alginate Nanoparticles
3.2.2 Optical Microscope
3.2.3 Dynamic Light Scattering
3.2.4 Zeta Potentail
3.2.5 Scanning Electron Microscopy
3.2.6 Transmission Electron Microscopy
3.2.7 Differential Scanning Calorimetry
3.2.8 In Vitro Drug Release Study
3.2.9 SDS-PAGE
3.2.10 Confocal Laser Microscopy
4 Results and Discussion
4.1 Preraration of Calcium alginate Nanoparticles
4.2 Formulation of W/O Microemulsion
4.3 Ternary Phase Diagrams
4.4 Charanterization of Calcium alginate Nanoparticles
4.4.1 Particle Size Analysis
4.4.2 Zeta Potential Measurements
4.4.3 Surface Morphology of Calcium alginate Nanoparticles
4.5 Characterization of Calcium alginate Nanoparticles loaded with BSA
4.5.1 Particle size analysis of BSA- Calcium alginate
4.5.2 Zeta Potential of BSA loaded Alginate Nanoparticles
4.5.3 Morphology of BSA loaded Nanoparticles
4.5.4 DSC of BSA loaded Calcium alginate Nanoparticles
4.5.5 BSA release profile
4.5.6 SDS-PAGE
4.5.7 Confocal Laser Scanning Microscopy of BSA loaded particles
4.6 Characterization of Chitosan Coated Ca-Alg nanoaprticles
4.6.1 Zeta Potential of Chitosan coated Ca-Alg Nanoparticles
4.6.2 Confocal Laser Microscopy of Chitosan Coated Ca-Alg microparticles
Conclusion
ReferencesBài viết liên quan
